Coding

Part:BBa_K2933010:Design

Designed by: Wenhui Gong   Group: iGEM19_TJUSLS_China   (2019-09-07)


subclass B1 metallo-beta-lactamase MUS-2, codon optimized in E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized in E. coli BL21(DE3).


Source

NCBI

References

MUS-2, a novel variant of the chromosome-encoded β-lactamase MUS-1, from Myroides odoratimimus. New Microbes New Infect. 2015 Jun 27;7:67-71. doi: 10.1016/j.nmni.2015.06.007. eCollection 2015 Sep. Al-Bayssari C1, Gupta SK2, Dabboussi F3, Hamze M3, Rolain JM2. NCBI PMID: 26257915